At The Benchside...
Molecular Biology & Biochemistry
- PCR & RT-PCR
- Agarose and AA gel electrophoresis
- GST-fusion protein purification
- Plasmid DNA manipulation: design, creation and propagataion of plasmid DNA
- Isolation of plasmid DNA, genomic DNA and total RNA
- Light Spectrometry - Potein and nucleic acid quantification
- genotyping from tail-DNA
Cell Biology
- Western Blot
- Immuno-Precipitation(IP) & Co-IP
- ImmunoFluorescence
- MTT-based toxicology - cell viabilty assay
- Fluorescent Microscopy
Cell Culture
- Basic Maintenance - passage, cryopreservation
- Experience gained on HeLa - L929 - Sf9 - LnCaP - SerW3 lines
- Isolation & Immortalizaton(3T3 protocol) of primary Mouse Embryo Fibroblasts
- Size-dependent isolation of male germ cells - Elutriation
- Transient & Stable transfection using cationic lipids or CaCl2
...And In The Office
- Medline and Internet searching using Pubmed / Google / Knowledgebases
- Microsoft Office & OpenOffice Office Suites
- Image Editing using Adobe Photoshop - Corel PhotoPaint - The Gimp
- Vector Graphics Based Drawing - InkScape & CorelDraw
- Python Scripting - able to automatize simple, repetitive tasks
- Can solve those frequent and annoying "Why doesn't it work" problems linked to informatics
